RESUMO
OBJECTIVE: To investigate the presence of Helicobacter pylori in a Colombian population. MATERIAL AND METHODS: Gastric biopsies from 60 patients with benign gastric pathologies were submitted to histopathological examination and genotyped by PCR through amplification of babA2 and iceA genes. RESULTS: The presence of H. pylori was demonstrated in 78.3%, 63.3% and 66.7% of the samples after Giemsa staining, andH. pylori 16S DNAr and iceA gene amplification, respectively. In addition, H. pylori babA2 positive was found infecting 7 patients. Among the iceA positive samples, 35% were identified as iceAl, 47.5% as iceA2 and 17.5% contained iceAl and iceA2 or different iceA2 types. This work allowed detection and genotyping of H. pylori, demonstrating a low proportion of patients carrying strains babA2 positive and an iceA genotype distribution according to previous reports. No association was found between the presence of babA2 and iceA genes and ulcer disease.
Assuntos
Adesinas Bacterianas/genética , Proteínas da Membrana Bacteriana Externa/genética , Gastrite/microbiologia , Infecções por Helicobacter/microbiologia , Helicobacter pylori/genética , Úlcera Gástrica/microbiologia , Biópsia , DNA Bacteriano/genética , Feminino , Gastroscopia , Genótipo , Helicobacter pylori/isolamento & purificação , Helicobacter pylori/patogenicidade , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Fatores de Virulência/genéticaRESUMO
Objective: To investigate the presence of Helicobacter pylori in a Colombian population. Material and Methods: Gastric biopsies from 60 patients with benign gastric pathologies were submitted to histopathological examination and genotyped by PCR through amplification of babA2 and iceA genes. Results: The presence of H. pylori was demonstrated in 78.3 percent, 63.3 percent and 66.7 percent of the samples after Giemsa staining, andH. pylori 16S DNAr and iceA gene amplification, respectively. In addition, H. pylori babA2 positive was found infecting 7 patients. Among the iceA positive samples, 35 percent were identified as iceAl, 47.5 percent as iceA2 and 17.5 percent contained iceAl and iceA2 or different iceA2 types. This work allowed detection and genotyping of H. pylori, demonstrating a low proportion of patients carrying strains babA2 positive and an iceA genotype distribution according to previous reports. No association was found between the presence of babA2 and iceA genes and ulcer disease.
Objetivo: El presente estudio investiga la presencia de Helicobacter pylori en pacientes de una población colombiana con enfermedad gastro-duodenal benigna y realiza su genotipificación usando como blanco los genes babA2 e iceA. Pacientes y Métodos: Se analizaron biopsias gástricas de 60 pacientes usando histopatologíay RPC. Resultados: La presencia de H. pylori se demostró en 78,3 por ciento, 63,3 por ciento y 66,7 por ciento de los pacientes, mediante tinción de Giemsa, amplificación del gen 16S ADNr y del locus iceA, respectivamente. Helicobacter pylori babA2 positivo se encontró infectando 7 pacientes y de las 40 muestras positivas para la presencia del locus iceA, 35 por ciento eran iceAl, 47.5 por ciento iceA2 y en 17,5 por ciento se evidenció infección múltiple. Se aportan datos sobre la prevalencia de H. pylori encontrando una baja proporción de casos babA2 positivos y una distribución de los genotipos iceA acorde con datos reportados previamente. La presencia de los genes babA2 e iceA no se encontró asociada con la presentación de úlcera.
